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TSB4

TSB4 refers to a specific formulation of Tryptic Soy Broth (TSB) supplemented with 4% Tween 20 and 0.5% Lecithin, a microbiological media used primarily for environmental monitoring, cleanroom validation, and sterility testing in pharmaceutical, food, and healthcare facilities. The Tween 20 and Lecithin act as neutralizers for residual disinfectants and sanitizers, allowing accurate recovery of microorganisms from surfaces or equipment after cleaning procedures. This media is supplied as a sterile, ready-to-use liquid in various volumes (90 mL, 190 mL, 490 mL, 500 mL, 990 mL, and 1 L) with a 180-day shelf life from date of manufacture. The product is manufactured under controlled conditions to ensure batch-to-batch consistency and is typically used in conjunction with standard microbiological testing protocols such as USP <61>, <62>, or ISO 14698. 3G Electric stocks multiple SKUs of TSB4 media, each identified by distinct manufacturer part numbers (e.g., 8WM-1180-90, 16WM-1180-190, 32WM-1180-490, 32WM-1180-500, 64WM-1180-990, 64WM-1180-1000), enabling procurement specialists to select the exact volume required for their testing workflows. The media is packaged in containers suitable for direct use in spray bottles, pour plates, or swab rinsing, depending on the application. As a distributor, 3G Electric provides datasheets with detailed specifications including lot numbers, expiration dates, and storage conditions (typically 2–30°C, away from light). Logistics support covers global shipping with temperature-controlled options where necessary to maintain media integrity. For plant engineers and maintenance managers overseeing cleanroom environments or aseptic processing areas, TSB4 media is a standard tool for verifying the efficacy of sanitation protocols. Procurement specialists can compare specifications across SKUs to match volume requirements and testing frequency, reducing waste and ensuring compliance with internal quality standards.

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About TSB4

TSB4 refers to a specific formulation of Tryptic Soy Broth (TSB) supplemented with 4% Tween 20 and 0.5% Lecithin, a microbiological media used primarily for environmental monitoring, cleanroom validation, and sterility testing in pharmaceutical, food, and healthcare facilities. The Tween 20 and Lecithin act as neutralizers for residual disinfectants and sanitizers, allowing accurate recovery of microorganisms from surfaces or equipment after cleaning procedures. This media is supplied as a sterile, ready-to-use liquid in various volumes (90 mL, 190 mL, 490 mL, 500 mL, 990 mL, and 1 L) with a 180-day shelf life from date of manufacture. The product is manufactured under controlled conditions to ensure batch-to-batch consistency and is typically used in conjunction with standard microbiological testing protocols such as USP <61>, <62>, or ISO 14698. 3G Electric stocks multiple SKUs of TSB4 media, each identified by distinct manufacturer part numbers (e.g., 8WM-1180-90, 16WM-1180-190, 32WM-1180-490, 32WM-1180-500, 64WM-1180-990, 64WM-1180-1000), enabling procurement specialists to select the exact volume required for their testing workflows. The media is packaged in containers suitable for direct use in spray bottles, pour plates, or swab rinsing, depending on the application. As a distributor, 3G Electric provides datasheets with detailed specifications including lot numbers, expiration dates, and storage conditions (typically 2–30°C, away from light). Logistics support covers global shipping with temperature-controlled options where necessary to maintain media integrity. For plant engineers and maintenance managers overseeing cleanroom environments or aseptic processing areas, TSB4 media is a standard tool for verifying the efficacy of sanitation protocols. Procurement specialists can compare specifications across SKUs to match volume requirements and testing frequency, reducing waste and ensuring compliance with internal quality standards.

Common Applications

  • Pharmaceutical cleanroom monitoring — TSB4 media (e.g., SKU 45H437, 1 L) for surface sampling after disinfection
  • Food processing facility hygiene testing — TSB4 media (e.g., SKU 45H434, 490 mL) for swab recovery of residual sanitizers
  • Medical device manufacturing — TSB4 media (e.g., SKU 45H432, 90 mL) for routine environmental monitoring of production areas
  • Disinfectant efficacy validation — TSB4 media (e.g., SKU 45H435, 500 mL) for neutralization assays per USP <1227>
  • Biotechnology research labs — TSB4 media (e.g., SKU 45H436, 990 mL) for sterility testing of equipment surfaces

Frequently asked questions

Common questions about TSB4 from 3G Electric.

What is the purpose of Tween 20 and Lecithin in TSB4 media?

Tween 20 (polysorbate 20) and Lecithin are added to Tryptic Soy Broth as neutralizers for residual disinfectants such as quaternary ammonium compounds, phenolics, and alcohols. In environmental monitoring, surfaces are sampled after cleaning; any remaining sanitizer could inhibit microbial growth, leading to false-negative results. The neutralizers bind or inactivate these chemicals, allowing viable microorganisms to grow in the broth. This formulation is specified in pharmacopeial methods like USP <1227> for recovery studies.

What is the shelf life of TSB4 media supplied by 3G Electric?

The TSB4 media stocked by 3G Electric has a 180-day shelf life from the date of manufacture. This is indicated on each container label along with the lot number and expiration date. Storage conditions should be maintained at 2–30°C, protected from direct light. Users should verify the expiration date upon receipt and avoid using media beyond that date to ensure reliable test results.

What volumes of TSB4 media are available through 3G Electric?

3G Electric offers TSB4 media in six volumes: 90 mL, 190 mL, 490 mL, 500 mL, 990 mL, and 1 L. Each volume corresponds to a specific manufacturer part number (e.g., 8WM-1180-90 for 90 mL). This range allows laboratories to select the appropriate size for their sampling protocols, whether for small-scale swab tests or larger surface rinse applications.

Is TSB4 media sterile and ready to use?

Yes, the TSB4 media supplied by 3G Electric is a sterile, ready-to-use liquid. It is manufactured under aseptic conditions and packaged in sealed containers. No additional preparation or sterilization is required before use. Users should follow standard aseptic technique when opening and dispensing the media to maintain sterility.

What applications is TSB4 media typically used for?

TSB4 media is commonly used for environmental monitoring in cleanrooms, isolators, and controlled environments, particularly in pharmaceutical, biotechnology, and medical device manufacturing. It is also employed in disinfectant efficacy testing and validation of cleaning procedures. The neutralizers allow recovery of bacteria and fungi that may be present on surfaces after sanitization.

Does 3G Electric supply TSB4 products?

Yes — 3G Electric (S) Pte. Ltd. supplies TSB4 products. We stock the TSB4 catalog and supply genuine factory-original products with full manufacturer warranty and technical support.