TSB4
Frequently asked questions
Common questions about TSB4 from 3G Electric.
What is the composition of TSB4 microbiology media?
TSB4 microbiology media are liquid Tryptic Soy Broth formulations containing 4% Tween 20 and 0.5% Lecithin. Tryptic Soy Broth is a general-purpose medium that supports the growth of a wide range of microorganisms. Tween 20 and lecithin are added to neutralize residual disinfectants that may be present on surfaces or in samples, which can otherwise inhibit microbial recovery. This composition is consistent across all SKUs in the TSB4 line, regardless of volume. The media are supplied as ready-to-use liquids with a 180-day shelf life. They are intended for environmental monitoring and surface sampling applications where disinfectant neutralization is required.
What sizes are available for TSB4 Tryptic Soy Broth with Tween 20 and Lecithin?
TSB4 Tryptic Soy Broth with 4% Tween 20 and 0.5% Lecithin is available in six sizes: 90 mL, 190 mL, 490 mL, 500 mL, 990 mL, and 1 L. Each size corresponds to a specific manufacturer part number, such as 8WM-1180-90 for 90 mL and 64WM-1180-1000 for 1 L. The range allows users to select volumes appropriate for different sampling methods, from small surface swabs to larger liquid samples. All sizes are supplied as liquids in ready-to-use containers. The 180-day shelf life applies to all volumes. 3G Electric stocks multiple SKUs to accommodate varying workflow requirements.
How does TSB4 media neutralize disinfectants?
TSB4 media contain 4% Tween 20 and 0.5% Lecithin, which are neutralizers that inactivate residual disinfectants. Tween 20 (polysorbate 20) is effective against quaternary ammonium compounds and phenolics, while lecithin neutralizes quaternary ammonium compounds and other lipophilic disinfectants. Together, they help prevent disinfectant carryover that could inhibit microbial growth in samples collected from surfaces or liquids. This neutralization is important for accurate environmental monitoring, as it allows recovery of microorganisms that might otherwise be suppressed. The concentrations used in TSB4 media are standard for this purpose. The media are designed for direct use in sampling and testing protocols.
What is the shelf life of TSB4 microbiology media?
TSB4 microbiology media have a shelf life of 180 days from the date of manufacture. This applies to all sizes in the product line, including 90 mL, 190 mL, 490 mL, 500 mL, 990 mL, and 1 L. The media should be stored according to the manufacturer's recommendations, typically at room temperature or as specified on the label. Proper storage ensures that the medium remains stable and performs as expected. Users should check the expiration date before use and discard any media that has exceeded its shelf life. 3G Electric ships TSB4 media globally, and customers should consider transit time when planning orders to ensure sufficient remaining shelf life upon receipt.
Can TSB4 media be used for sterility testing?
TSB4 media are formulated for environmental monitoring and surface sampling, but they can also be used for sterility testing in some contexts. The presence of Tween 20 and lecithin makes them suitable for neutralizing disinfectants that might be present in samples. However, for compendial sterility testing, users should verify that the medium meets the requirements of the relevant pharmacopeia, such as USP <71> or EP 2.6.1. TSB4 media are not specifically certified for sterility testing unless stated otherwise. The product information does not include claims about compendial compliance. Users should validate the medium for their specific application. 3G Electric provides TSB4 media as a general-purpose neutralizer-containing TSB.
What industries use TSB4 microbiology media?
TSB4 microbiology media are used in industries that require environmental monitoring and microbial recovery, including pharmaceuticals, food and beverage, healthcare, and environmental testing. In pharmaceutical manufacturing, they are used for surface sampling in cleanrooms and controlled environments. In food and beverage, they help monitor hygiene in production areas. Healthcare facilities may use them for environmental surveillance. Environmental testing laboratories use them for surface and water sampling. The neutralizer-containing formulation is particularly useful where disinfectants are routinely applied. TSB4 media are available in multiple volumes to suit different sampling scales. 3G Electric supplies these products globally to support quality control and monitoring programs.
